Steady state kinetic parameters mAOX4 wildtype and variants with different substrates.
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Steady-state kinetics were corrected to molybdenum saturation of 100%. Kinetic parameters were recorded in 50 mM Tris-HCl, 200 mM NaCl, and 1 mM EDTA (pH 8.0) in the presence of 100 μM DCPIP as electron acceptor. Substrate concentration were varied around 0.5 and 10 times the KM. Data are mean values from three independent measurements (±S.D.).
创建时间:
2018-01-26



