Characterizing resistance to Soybean Cyst Nematode in PI 494182, an early-maturing soybean accession

In this work, we performed a quantitative trait locus (QTL) analysis of the SCN-resistant soybean accession PI 494182 (MG 0). A RIL population (Costaud x PI 494182) segregating for SCN resistance was challenged with SCN (Hg type 0) and genotyped via genotyping by sequencing (GBS) to produce a genetic map. A subset of the RIL population was confronted to a Hg type 2.5.7 SCN population and some of these exhibited resistance towards this type. Whole-genome sequencing of PI 494182 and Costaud allowed us to determine the resistance alleles and their copy number. We also used agronomic phenotypic data to determine the impact of selecting for PI 494182 alleles at some SCN-resistance QTLs on protein content, oil content, 100-seed weight and maturity time. This work provides useful markers for introgressing SCN resistance in early-maturing soybean varieties.