Transcriptomic Analysis of Differentiating Primary Myoblasts treated or not with TGFB1 recombinant protein or ITD-1 compound.

Transforming Growth Factor beta (TGFβ) signaling cascade plays a fundamental role during muscle development and regeneration. Previous reports proposed several roles for the TGFβ superfamily members and generally they are considered negative regulators of myogenic differentiation. However, the activation of this signaling pathway has been shown to be essential to obtain a correct muscle tissue repair process. Due to these contrasting results, we decided to investigate the role of TGFb cascade in muscle cells. To identify the genetic networks regulated by TGFB signaling in muscle cells, we performed transcriptome analysis of differentiated primary myocytes, stimulated with either TGFB1 or ITD-1 We cultured primary myoblasts at 30,000 cells per cm2 onto matrigel-coated plates. Once adherent, cells were incubated in differentiation medium (DMEM with 2% Horse serum and 1% penicillin/streptomycin) for 24 hours. At this point, myoblasts were treated with TGFB1 recombinat protein at a final concentration of 20ng/ml or ITD-1 compound at 5mM for 24 hours.