S1 SNPS.xlsx

<b><i>SNPs estimates for heterozygosity</i></b>Using SNPs molecular markers for heterozygosity, the S₁ generation showed a good proportion of heterozygotes at several loci across the genome, indicating that heterozygosity is very high in cassava, and several selfing cycles may be required to reduce it drastically. SNP analysis revealed percentage heterozygosity between 12% and 65% in the population. The results showed that estimates of the heterozygosity index tended to increase and improve as a greater number of SNP markers were used. This suggests that an optimum number of SNPs should be determined to improve the precision of heterozygosity estimates in cassava. The findings also demonstrated that a majority of the progenies, specifically over 55%, exhibited heterozygosity values below 25%. This suggests that there are quality materials to pass on to the next inbreeding generation, which will likely result in less heterozygosity in the subsequent breeding generation.