Periplasmic stress contributes to a tradeoff between protein secretion and cell growth in E. Coli Nissle

To investigate changes in E. coli Nissle 1917 gene expression when engineered to secrete a large amount of protein via the curli (Type VIII) secretion system. Overall design: To investigate variants of protein secretion and conduct gene expression profiling analysis to determine genes that are upregulated due to cellular burden from protein secretion. The different variants derived include: no protein expression or secretion, localized protein expression in the cytoplasm, and protein expression with secretion to the extracellular space (i.e., secretion through the inner and outer membrane).