Pyruvate–GPR31 axis induces LysoDC dendrite protrusion to M-cell pockets for effective immune responses; Supplementary videos

Name: Pyruvate–GPR31 axis induces LysoDC dendrite protrusion to M-cell pockets for effective immune responses; Supplementary videos
Creator: Umemoto, Eiji; Nakanishi, Katsuhiro
Published: 2025-04-29T00:00:00+08:00

Figshare2025-04-29 更新2026-04-08 收录

肠道粘膜免疫成像

免疫细胞动态行为分析

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https://figshare.com/articles/dataset/_b_Pyruvate_GPR31_axis_b_b_induces_LysoDC_dendrite_protrusion_to_M-cell_pockets_for_effective_immune_responses_Supplementary_videos_b_/28888943/1 数据链接链接失效反馈

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Supplementary Video 1. Two-photon microscopic imaging of a Peyer's patch (PP) explant prepared from untreated Gpr31b+/+ Cx3cr1gfp/+ mice. PP explants prepared from untreated Gpr31b+/+ Cx3cr1gfp/+ mice were stained with anti-GP2 antibody (red) and vertically monitored with a z-step interval of 0.5 µm by two-photon microscopy. Arrows indicate LysoDC dendrites in M-cell pockets. The arrows remain in place after the image has moved to a depth where the dendrites are not visible. Scale bar, 50 µm. Video corresponds to Figure 2(a).Supplementary Video 2. Two-photon microscopic imaging of a PP explant prepared from untreated Gpr31b-/- Cx3cr1gfp/+ mice. PP explants prepared from untreated Gpr31b-/- Cx3cr1gfp/+ mice were stained with anti-GP2 antibody (red) and vertically monitored with a z-step interval of 0.5 µm by two-photon microscopy. Arrows indicate LysoDC dendrites in M-cell pockets. The arrows remain in place after the image has moved to a depth where the dendrites are not visible. Scale bar, 50 µm. Video corresponds to Figure 2(a).Supplementary Video 3. Two-photon microscopic imaging of a PP explant prepared from untreated Gpr31b+/+ Cx3cr1gfp/gfp mice. PP explants prepared from untreated Gpr31b+/+ Cx3cr1gfp/gfp mice were stained with anti-GP2 antibody (red) and vertically monitored with a z-step interval of 0.5 µm by two-photon microscopy. Arrows indicate LysoDC dendrites in M-cell pockets. The arrows remain in place after the image has moved to a depth where the dendrites are not visible. Scale bar, 50 µm. Video corresponds to Figure 2(a).Supplementary Video 4. Two-photon microscopic imaging of a PP explant prepared from pyruvate-treated Gpr31b+/+ Cx3cr1gfp/+ mice. PP explants prepared from pyruvate-treated Gpr31b+/+ Cx3cr1gfp/+ mice were stained with anti-GP2 antibody (red) and vertically monitored with a z-step interval of 0.5 µm by two-photon microscopy. Arrows indicate LysoDC dendrites in M-cell pockets. The arrows remain in place after the image has moved to a depth where the dendrites are not visible. Scale bar, 50 µm. Video corresponds to Figure 2(a).Supplementary Video 5. Two-photon microscopic imaging of a PP explant prepared from pyruvate-treated Gpr31b-/- Cx3cr1gfp/+ mice. PP explants prepared from pyruvate-treated Gpr31b-/- Cx3cr1gfp/+ mice were stained with anti-GP2 antibody (red) and vertically monitored with a z-step interval of 0.5 µm by two-photon microscopy. Arrows indicate LysoDC dendrites in M-cell pockets. The arrows remain in place after the image has moved to a depth where the dendrites are not visible. Scale bar, 50 µm. Video corresponds to Figure 2(a).Supplementary Video 6. Finger-like dendrite of LysoDCs in M-cell pockets. PP explants prepared from untreated Gpr31b+/+ Cx3cr1gfp/+ mice were monitored as described in Supplementary video 1. Volume rendering-based 3D reconstruction of the LysoDC protruding a finger-like dendrite into an M-cell pocket is shown. Video corresponds to Figure 2(e).Supplementary Video 7. Spherical dendrite of LysoDCs in M-cell pockets. PP explants prepared from pyruvate-treated Gpr31b+/+ Cx3cr1gfp/+ mice were monitored as described in Supplementary video 4. Volume rendering-based 3D reconstruction of the LysoDC protruding a spherical dendrite into an M-cell pocket is shown. Video corresponds to Cell #2 in Figure 2(g).

提供机构：

Umemoto, Eiji; Nakanishi, Katsuhiro

创建时间：

2025-04-29