Microarray profiling of YTHDF2 deficient mouse oocytes

To study the role of the protein YTHDF2 during female gametogenesis, its gene was conditionally deleted in oocytes. Total RNA samples from YTHDF2 deficient GV and MII oocytes (and control oocytes) were subjected to array profiling.为探究蛋白质YTHDF2在雌性配子发生过程中的作用，研究人员在卵母细胞中对该基因实施了条件性敲除。针对YTHDF2缺陷型GV（生发泡）期、MII（第二次减数分裂中期）期卵母细胞及对照卵母细胞的总RNA样品，研究人员开展了芯片表达谱分析。