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Auxin repressors expressed in maize inflorescence exhibited variable auxin-induced degradation dynamics. D, The 16 ZmIAAs coexpressed with Arabidopsis TIR1 were degraded in response to auxin. Fluorescence measurements were obtained 2 h post-auxin exposure on a flow cytometer. Data from two replicates are shown; error bars represent se. AU, Absorbance units. Conclusion: D, The 16 ZmIAAs coexpressed with Arabidopsis TIR1 were degraded in response to auxin. Fluorescence measurements were obtained 2 h post-auxin exposure on a flow cytometer. Data from two replicates are shown; error bars represent se. AU, Absorbance units. Yeast autofluorescence was cancelled out using non-fluorescent yeast. All yeast strains were tested for basal fluorescence prior to treatment from over 50 minutes of growth.

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2020-06-01
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