Bioinformatic analysis of candidate genes.

aReports the GenBank accession number of the sequence with the lowest e value.
bReports the species with the most similar homolog; Is = I. scapularis, Tc = Tribolium castaneum, Av = Amblyomma variegatum.
cIndicates whether the alignment with a known protein indicates the presence of the first coding amino acid in the cDNA sequence; Y = yes; N = no.
dIndicates whether there are any recognized transmembrane domains using TMpred; Y = yes; N = no.
eIndicates the presence of a signal peptide; Y = yes; N = no. Note: if No is indicated in the 5′ end column, the positive SP prediction may actually reflect the presence of a TM domain near the start of the sequence rather than a true signal peptide.