Gene expression profiling of neural crest progenitor cultures derived from human embryonic stem cells carrying nonsense mutations in the Polycomb gene ASXL1 [HOM]
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
下载链接：
http://data.iscr.ac.cn/Article?id=912fd661fcf4bd5e0706697fb9d3d2c4
下载链接
链接失效反馈官方服务：
资源简介：
We observed impaired neural crest cell differentiation from human embryonic stem cells that harbor nonsense mutations in the Polycomb gene ASXL1. To investigate the underlying molecular mechanisms, we harvested neural crest differentiation cultures from wildtype and homozygous ASXL1 mutant lines at day 7 of a neural crest differentiation protocol and performed total RNA sequencing using the Illumina NextSeq system. Analyses of global transcriptomes revealed profound changes between wildtype and mutant cultures, and we identified downregulation of several genes of the neural crest regulatory network, most prominently of the transcription factor ZIC1.
提供机构：
Helmholtz Center Munich
创建时间：
2022-02-20
