Analysis of changes in gene expression in skin epidermis upon conditional knockout of PRC2-subunit Eed

Analysis of changes in gene expression in skin epidermis upon conditional knockout of the essential Polycomb repressive complex 2 (PRC2) subunit Eed. Loss of Eed in skin epithelium leads to de-repression of key Merkel-differentiation genes, which are known PRC2 targets, and results in ectopic formation of Merkel cells that are associated with all hair types. Gene expression analysis: To determine the changes in gene expression in skin epidermis upon conditional knockout of Eed, total RNA was isolated from skin epidermis in four biologic replicates from cells in different conditions and hybridized to SurePrint G3 Mouse GE 8X60K microarrays (Agilent).皮肤表皮中关键多梳抑制复合体2（Polycomb repressive complex 2, PRC2）亚基Eed条件性敲除后的基因表达变化分析。皮肤上皮内Eed的缺失会导致关键梅克尔细胞（Merkel cells）分化基因的去抑制，这类基因属于已知的PRC2靶标，同时会引发与所有毛发类型相关的梅克尔细胞异位生成。基因表达分析：为明确Eed条件性敲除后皮肤表皮的基因表达变化，研究人员从不同培养条件下的细胞中提取皮肤表皮的总RNA，设置4次生物学重复，并将样本与SurePrint G3小鼠基因表达8×60K微阵列芯片（Agilent）进行杂交。