Additional file 6 of A detached leaf assay for testing transient gene expression and gene editing in cowpea (Vigna unguiculata [L.] Walp.)

Additional file 6: Table S1. Genetic variation between two cowpea accessions (IT97K-499-35 and IT86D-1010) using Spriggs et al. [40] and Lonardi et al. [26] genome resources for comparison. Table S2. List of genes identified in this study. Table S3. Color codes and descriptions for gene families in microsyntenic region spanning 5 genes upstream and downstream of gene of interest. Table S4. SPO11-1, REC8 and OSD1 protein pairwise identities (in percentages) of legume species relative to cowpea. Table S5. Splice variant analysis of SPO11-1, REC8 and OSD1 genes in IT86D-1010 genotype. Table S6. Gene structure of SPO11-1, REC8 and OSD1 genes in legumes and Arabidopsis. Table S7. Normalized read count of SPO11-1, REC8 and OSD1 genes in laser-captured cell-type datasets. Table S8. Cowpea U6 small nucleolar RNAs non-coding genes identified in this study. Table S9. Edits detected in SPO11-1, REC8 and OSD1 genes via the CRISPR/Cas9 transient assay in detached cowpea leaves. Table S10. Edits detected in SPO11-1-targeted T0 transgenic lines. Table S11. List of primers.