RNA sequencing analysis of MCF7 cells treated with several combinations of steroids
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https://www.ncbi.nlm.nih.gov/sra/SRP316341
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资源简介：
We report the transcriptomic profile induced on MCF7 cells by several combinations of steroids (estetrol, estradiol, progesterone, R5020). Overall design: Routinely cultured MCF7 cells were treated in red phenol-free DMEM medium, supplemented with 2% heat-inactivated and dextran-coated charcoal- treated FBS with estradiol (10-9M), estetrol (10-7M or 10-10M) or vehicle (DMSO 0.001%) for 20h, then progesterone (10-7M) or R5020 (10-8M) were added in some conditions for 4 additional hours. After 24h total treatment, RNA was extracted, quantified and submitted to RNAseq analysis.
创建时间：
2021-07-16
