Bulk RNA sequecing reveals the heterogeneity of fibroblasts in the diabetic heart

Diabetes mellitus is one of the most chronic diseases, of which diabetic cardiomyopathy is the major cause of morbidity and involves in multiple processes such as inflammation, oxidative stress, fibrosis, extracellular collagen deposition, apoptosis, mitochondria dysfunction. Overall design: We established diabetic mice by STZ injection and lead bulk RNA sequencing to reveal the potential mechanisms in diabetic myocardium. Real-time quantitative PCR, immunofluorescence and western blotting was used to verify the sequence data. In addition, cardiac function , fibrosis related indicators and morphological changes were performed to analyze the dysfunctions of diabetic cardiomyopathy.