Single-nucleus transcriptomic highlights sex differences in cardiac molecular signaling and identifies a novel cardiomyocyte population associated with CCNA2-mediated cardiac regeneration

Adult mammalian hearts lack regenerative potential and this is a significant contributing cause of the extensive morbidity and mortality of cardiovascular disease. We performed single nucleus RNA sequencing (snRNA-seq) to capture of cell diversity and gender-specific changes as well as identification of critical differentially expressed genes in cellular clusters throughout the heart. Adult hearts isolated from female and male normal wild-type (both nontransgenic-nTG and CCNA2 constitutively expressing-Transgenic-Tg) mice.成年哺乳动物心脏缺乏再生潜能，这是心血管疾病引发极高发病率与死亡率的重要诱因之一。本研究采用单细胞核RNA测序（single nucleus RNA sequencing, snRNA-seq）技术，捕获心脏全域的细胞多样性与性别特异性表达变化，并鉴定全心脏各细胞簇中的关键差异表达基因。实验所用成年心脏均分离自雌性与雄性正常野生型小鼠，涵盖非转基因（nontransgenic-nTG）及周期蛋白A2（CCNA2）组成型表达的转基因（Transgenic-Tg）两类品系。