Effects of signaling inhibitors on IL-32γ delayed apoptosis.
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https://figshare.com/articles/dataset/_Effects_of_signaling_inhibitors_on_IL_32_947_delayed_apoptosis_/1191316
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IL-32γ-delayed apoptosis is dependent on kinase activation. Neutrophils were pre-incubated 30 min with an inhibitor of PI-3 kinase (LY249002 at 20 µM), an inhibitor of MEK (U0126 at 10 µM), an inhibitor of p38 MAP kinase (SB202190 at 25 µM), and an inhibitor of JN Kinase (SP600125 at 10 µM) or their vehicle (DMSO). Neutrophils were then stimulated with IL-32γ (300 ng.ml−1) for 20 h and further analyzed by FACS to evaluate their annexin V-FITC binding in conjunction with propidium iodide staining. The effects of signaling inhibitors were compared to the experimental condition in which neutrophils were incubated with IL-32γ + inhibitor vehicle (DMSO); this condition corresponds to 100% of delayed apoptosis. Results are expressed as percentage of IL-32γ inhibitory effect. Results are means ± s.e.m. of 5–9 different donors. Statistics: paired Student t test, p values are indicated in the table. NS = non-significant.
Effects of signaling inhibitors on IL-32γ delayed apoptosis.
创建时间：
2014-10-02
