The role of RUNX2 and BHLHE40 in pathologically relevant CD4-positive tissue-resident T-cells in Crohn's disease. (RNA-seq, CD4+ T cell, part2)

Tissue-resident T-cells (TRM) are deeply involved in immune memory at the site of inflammation. Here, we identified two key transcription factors, RUNX2 and BHLHE40 as regulators of pathologically relevant CD4-positive TRM in the inflamed gut mucosa of Crohn's disease patients. Overall design: CD4+ CD103+ HLA-DR+ and HLA-DR- T cells were sorted from mononuclear cells derived from surgically resected fresh gut specimens by flow cytometry. Total RNA was extracted using TRIzol reagent and subjected to RNA-sequencing.