Distribution of osteoclasts during craniofacial development in wild-type zebrafish

Craniofacial development involves a wide variety of different cell types working in concert to generate adult structures. One of these cell types, the osteoclast, has not been well studied in the context of normal craniofacial development. Our aim in generating this series of imaging data was to visualize where osteoclasts aggregate and are distributed during development across multiple individuals. Through low-magnification live confocal imaging on size-matched juvenile zebrafish, we generated merged images of osteoclast distribution overlaid on a representative image of bone structure. This data encapsulates the majority of the skull through images from the dorsal, ventral and lateral sides. This merged images complement our datasets tracking osteoclasts in specific individuals over time.