B2 RNA enhances viral replication and gene expression.
收藏Figshare2016-02-23 更新2026-04-29 收录
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(A) NIH3T3 cells transfected with indicated ASOs were infected with MHV68 at an MOI of 0.05. Infection was allowed to progress for 72 h. Primer extension was performed at 24 hpi infection to monitor the levels of B1, B2, and 7SK RNA. (B) Infectious virus produced in (A) was quantified by plaque assay in NIH3T3 cells. (C) RNA isolated at 72 hpi was used to monitor the levels of viral and cellular RNAs by RT-qPCR. (D) ChIRP was performed on NIH3T3 cells infected with MHV68 for 24 h. Antisense LacZ oligos were used as a negative control. RNAs isolated from the ChIRP experiment were analyzed by small RNA northern blotting for B2 SINE and 5S rRNA. (E) DNA isolated from the ChIRP experiment described in (D) was used for qPCR.
创建时间：
2016-02-23
