Heparan sulfate chain conjugated laminin-E8 fragments support a xeno-free system for myogenic differentiation from human pluripotent stem cells. Homo sapiens

We describe a system for differentiating hiPSCs on new generation laminin fragments (NGLFs), a recombinant form of a laminin E8 fragment conjugated to the heparan sulfate chain (HS) attachment domain of perlecan. Depending on its unique structure, HS in NGLF binding to fibroblast growth factors (FGFs) strongly stimulates the FGF signaling pathway to trigger hiPSC differentiation, subsequently increasing the population of the primitive streak, paraxial mesoderm cells, myocytes, and muscle stem cells. In this project, we sampled cells cultured on Matrigel (MG), LM421-E8 (421) or NGLFs (p421) at Day 0, Day2, Day4 and Day14 of differentiation, then performed RNA-seq and transcriptome analysis.