Multi-organ transcriptomic profiling of short-term fasted mice

To investigate the global gene expression dynamics associated with short-term fasting, we used mRNA-seq to profile the transcriptomes of nine organs obtained from mice subjected to six different STF duration (0, 2, 8, 12, 18 and 22 hours of fasting; n=3 per time point; Fig. 1a). The nine organs profiled were: olfactory bulb (OB), brain (BRN, which includes the telencephalon and diencephalon), cerebellum (CBL), brainstem (BST, which consists of the mesencephalon, pons, and myelencephalon), stomach (STM), liver (LIV), interscapular brown adipose tissue (iBAT), perigonadal white adipose tissue (pgWAT), and posterior-subcutaneous white adipose tissue (psWAT). mRNA profiling of nine organs obtained from wild type (WT) mice subjected to six different short term fasting duration