CUT&Tag profiling of H3K18LA modification in rat pancreas with acute pancreatitis

This study explores histone modification dynamics in acute pancreatitis. Sprague-Dawley rats were intraperitoneally injected twice with L-arginine (2.5 g/kg, 1-hour interval) to induce acute pancreatitis. Pancreatic tissues were harvested after 48 hours. CUT&Tag was performed using anti-H3K18LA antibody (IP) and corresponding input control to assess promoter-associated chromatin changes in the inflamed pancreas. Overall design: Acute pancreatitis was induced in Sprague-Dawley rats by two intraperitoneal injections of L-arginine (2.5 g/kg) with a 1-hour interval. After 48 hours, pancreatic tissue was collected. CUT&Tag was performed using anti-H3K18LA antibody to identify histone modification patterns related to promoter activity. Input DNA from the same samples was used as control. The goal was to evaluate H3K18LA enrichment in the inflamed pancreas.