Agilent 8x60K Custom Exon array of human temporal cortical tissue samples with varying degree of Alzheimer's Disease (AD)-related neurofibrillary pathology

In this study, we coupled microarray-based transcriptomics and MS-based phosphoproteomics assay to determine mRNA, protein, and phosphopeptide expression levels from 71 autopsied temporal cortical samples, with varying degree of Alzheimer's Disease (AD)-related neurofibrillary pathology. With computational analysis, we identified disease-related transcript, protein and phosphopeptide expression patterns, associated with distinct biological processes and cell types. 71 human temporal cortical tissue samples were divided into 7 groups based on Braak staging (degree of AD-related neurofibrillary pathology) indicating the degree of disease severity. From 60 of these samples, transcripts were identified by microarray analysis. Additionally, utilizing liquid chromatography tandem mass spectrometry (LC-MS/MS) phospho-proteomics, proteins and phosphopeptides were identified from 36 samples (Data found in PRIDE). Furthermore, APOE genotype, Alpha-, beta-, and gamma-secretase activity, and amyloid-beta 42 levels were determined from the tissue samples.