Effects of gene scilencing of USP2 on expression profiles of HL60 cells (PMA 3days and LPS 4hrs)

So far, we have found phorbol 12-myristate 13-acetate (PMA) induced ubiquitin specific peptidase (USP) 2b isoform in myeloid leukemia cell lines such as HL60, THP-1, and U937. HL60, THP-1, and U937 undergoes differentiation into macrophage-like cells after stimulation with phorbol ester. To explore molecular function of USP2 in macrophages especially during lipopolysaccharide(LPS) response, we assess expression profiles of HL60-derivatives continuously expressing shRNA for USP2 and control shRNA. HL60 derivatives continuously expressing shRNA for USP2 and control shRNA were treated with PMA (30nM) for 3 days, and subsequently stimulated with E. coli LPS for 4 hrs. Total RNA was extracted from the cells and subjected to microarray experiments using Human Genome U133 GeneChips.