Identifying interferon-stimulated genes enriched in multiple tissues of Irgm1-/- mice

We observed enhanced epithelial proliferation secondary to elevated Type I IFNs in multiple tissues of Irgm1-/- mice. We discovered that Type I IFNs signaled through macrophages to promote this epithelial hyperproliferation. To determine candidate factors that could play a role in this process, we performed whole genome microarray analysis of salivary gland, small intestine, and isolated colonic macrophages of Irgm1-/- mice and C57BL/6 WT controls. Genes enriched in at least 2 of the 3 sample sources were then screened for the ability to augment epithelial proliferation in vitro. RNA was extracted from three (small intestine and isolated colonic macrophage samples) or four (salivary gland samples) mice of each genotype (Irgm1-/- and WT). Both male and female six to nine week-old mice were used.我们在Irgm1基因敲除（Irgm1-/-）小鼠的多个组织中观察到，上皮增殖因I型干扰素（Type I IFNs）水平升高而增强。本研究发现，I型干扰素通过巨噬细胞介导信号通路，促进上述上皮过度增殖。为筛选在此过程中发挥潜在作用的候选因子，我们对Irgm1-/-小鼠与C57BL/6野生型（WT）对照小鼠的唾液腺、小肠及分离的结肠巨噬细胞开展了全基因组微阵列分析。随后对在至少2种样本来源中富集的基因进行体外上皮增殖增强能力的筛选。每种基因型（Irgm1-/-与WT）的小鼠中，小肠及分离的结肠巨噬细胞样本取自3只小鼠，唾液腺样本则取自4只小鼠。实验使用6至9周龄的雌雄小鼠。