genome editing using type I-E CRISPR-Cas3 in mice and rat zygotes

we introduce a novel genome editing strategy in zygotes using the type I-E CRISPR-Cas3 system, which efficiently generates deletions of several thousand base pairs at targeted loci in mice without off-target mutations. To overcome the difficulties associated with detecting the variable deletions, we used a newly long-read sequencing-based multiplex genotyping approach using DAJIN2.