RNA Sequencing analysis of xenografts for mouse gene expression changes

Control and 2G8-treated MiaPaca-2 and Colo375 xenografts were subjected to RNA-seq analysis for mouse gene expression changes Overall design: Tumor tissues were harvested in RLT lysis buffer and purified according to instructions of the RNeasy Plus Kit (QIAGEN). RNA concentration was determined by a Qubit fluorometer (Thermo Fisher Scientific) and then prepared with TruSeq Stranded Total RNA Sample Prep Kit (Illumina). Samples were quantified, normalized, and sequenced on the Illumina HiSeq 2500 with at least 25 million reads per sample. FASTQ files were aligned to mouse mm10 reference transcriptome.