MSH1 disruption in plants triggers RdDM-mediated reprogramming of phenotypic plasticity (Bisulfite-Seq I)

To generate epi-lines, two third-generation msh1 T-DNA mutant (SAIL_877_F01) plants were used to pollinate Col-0 to generate two independent F1 populations. Derived F1 progenies were self-pollinated to generate F2 populations that were genotyped for the msh1 T-DNA mutation. MSH1 F2 plants were self-pollinated to produce F3 families that were used in the study. Overall design: 3 plants from each of the 4 epi populations and Col-0 WT control were sequenced.