In vivo analysis of circRNA expression alterations in response to influenza virus infection. In vivo analysis of circRNA expression alterations in response to influenza virus infection

Our study's objective was to determine circRNA expression alterations following in vivo influenza virus infection. We challenged C57BL/6 for 48 hours with IAV-PR8. Lung samples were obtained, RNA-Seq was utilized, and the circRNAs transcriptome data was compared to the control. Overall design: Ten SPF C57BL/6 mice were divided into two groups (mock-infected and IAV PR8 infected). The infected group was inoculated with 1x104 PFU/ml of IAV PR8 viral stock through intranasal instillation. Mice were euthanized, and lung samples were collected 48 hours post-infection (hpi). Mice lungs were sent for circRNAs transcriptome analysis RNA-Seq by Shanghai OE Biotech Co., Ltd. (Shanghai, China) using an Illumina NovaSeq 6000 instrument.