Divergent immunometabolic changes in adipose tissue and skeletal muscle with ageing in healthy humans. Divergent immunometabolic changes in adipose tissue and skeletal muscle with ageing in healthy humans

RNA-sequencing from 12 young (20-35 years old) and 12 older (60-85 years old) participants' skeletal muscle and adipose tissue. Biopsies were taken at rest following an overnight fast, and following alcohol/caffeine restriction for 24 hours, and no strenuous physical activity for 48 hours. Skeletal muscle was taken by the Bergstrom technique under local anaesthesia (Lidocaine hydrochloride) from the dominant leg Vastus Lateralis, and adipose tissue was taken by needle aspiration 5cm lateral to the umbilicus. Overall design: Examination of the effect of ageing on adipose tissue and skeletal muscle transcriptomes in healthy, active younger and older men