Transcriptome profile of fibroblast-like synoviocytes with FTO knockdown. Transcriptome profile of fibroblast-like synoviocytes with FTO knockdown

Fibroblast-like synoviocytes (FLS) are crucial in promoting articular inflammation and destruction in rheumatoid arthritis (RA). As the most abundant RNA modification, the function of m6A in RA FLS is still unclear. Here, we constructed FTO-knockdown FLS to explore the mechanism of FTO in regulating the aggressive behavior of RA FLS. Overall design: To investigate the function of FTO in RA FLS，FTO-knockdown FLS were established by shRNA-lentivirus infection. RNA sequencing was then performed to explore the specific downstream targets of FTO.