ATACseq of Naïve CD4+ cells from WT, Stat6, and Il21e KO mice

We report that Stat6-driven gene transcription regulates the transition from Tfh21 to Tfh4 cells, suppressing IL-21 production and promoting IL-4 secretion. We identified a cis-enhancer in the second intron of Il21 as a regulator of Tfh cell cytokine transition, via an effect of Stat6 on chromatin accessibility with repression of Il21 transcription. Chromatin accessibitiy of this second intron region was not affected in the naive CD4+ T cell population Overall design: Examination of naïve CD4+ T cells populations from WT, Stat6, and Il21e KO mice