Effects of X-ray irradiation and housing conditions on mitochondria in Peromyscus maniculatus

Challenging environmental conditions, such as exposure to ionizing radiation, can induce oxidative stress within mitochondria. However, organisms show variation in their capacity to cope with cellular stress. This study aimed to identify how housing conditions influence mitochondrial stress responses under exposure to radiation. We used North American deer mice (Peromyscus maniculatus), kept in standard rodent boxes or large semi-natural enclosures that allowed for natural levels of physical activity and social structures under ambient atmospheric conditions. Animals from each housing condition were exposed to 2000 mSv (2 Gy) of ionizing X-ray radiation, a dose comparable to long-term spaceflight. Animals were euthanized for analysis prior to irradiation (baseline), within four days post-irradiation, or one-month following irradiation. Relative mass of the liver was lower than the baseline for irradiated animals maintained in the semi-natural enclosures. State 4 and coupled and uncouple..., Data were collected from deer mice housed in boxes (3 groups) or large semi-natural enclosures (3 groups). Within each housing environment, data were collected from animals at baseline (before radiation exposure), 1-4 days after radiation exposure (days after), and one month after exposure. Liver and skeletal muscles were harvested from each deer mouse. For mitochondrial respiration measurements, mitochondria were isolated, and mitochondrial respiration was run on an Oroboros High-Resolution Respirometer. To quantity oxidative stress in each organ, TBARS and protein carbonyls were measured in each group. These data are presented as raw values in an Excel file. Note that there are multiple tabs in the file., , # Effects of X-ray irradiation and housing conditions on mitochondria in Peromyscus maniculatus

[https://doi.org/10.5061/dryad.fqz612k05](https://doi.org/10.5061/dryad.fqz612k05)

The data are presented in a single Excel file.

## Description of the data and file structure

Within the Excel file, all tabs include:

* each animal's unique '*animal tag*' number
* timing of '*irradiation group*' which occurred at baseline (before irradiation), 1-4 'days after' irradiation, or a 'month after' irradiation
* '*housing group*' - animals were housed in a 'box' or a semi-natural 'enclosure'
* '*sex*' - male or female
* '*box/enclosure nr*' - where animals were housed (different letters designate different enclosures, different number designate different boxes - each number or letter is arbitrary)

Tab 'Body_Mass'

* Body mass for each week at Auburn University is presented ('Body mass week 1', 'Body mass week 2', etc.), as well as the average of all data, 'Body Mass Average'.

Tab \"Liver_Respir...,