Nociceptor neurons control pollution-mediated neutrophilic asthma; Expression profile of vagal nociceptor neurons in air pollution-exacerbated allergic airway inflammattion

Sequencing of TRPV1+ neurons in jugular-nodose complex (JNC) in a mouse model of air pollution-exacerbated allergic inflammation. Overall design: TRPV1-cre/wt::tdTomato-fl/wt mice were sensitized via intraperitoneal injection with a mix of grade V OVA (200 µg/dose; Sigma-Aldrich A5503) and Imject® Alum (1 mg/dose; ThermoFisher 77161) on days 0 and 7. Subsequently, they underwent intranasal challenges with OVA (50 µg/dose), with or without fine particulate matter (FPM; 20 µg/dose; NIST 2786), from day 14 to 16. Control mice were sensitized but not challenged. The mice were euthanized on day 17, jugular-nodose complex (JNC) were collected, and tdTomato+ cells from naive, OVA-challenged, and OVA-FPM co-exposed mice were sroted by FACS.