Transcriptomic analysis of Drosophila larval lymph glands

The lymph gland is one of the main larval hematopoietic organin in Drosophila melanogaster. In wnandering third instar larav, it is composed of two pairs of anterior lobes, followed by 2 to 3 pair of posterior lobes. To gain further insight into the gene expression repertoire of Drosophila blood cells, we established the gene expression profiles of third instar larva lymph glands anterior and posterior lobes. Drosophila lymph gland anterior and posterior lobes were dissected from third instar larvae (white1118) and their RNA were extracted for analysis by deep sequencing, in triplicate, using Illumina NextSeq sequencing platform.