Set3: ERCC bulk Loop-seq long-read RNA-seq by Element Biosciences AVITI platform

DNA sequencing is a critical tool in modern biology. Over the last two decades, it has been revolutionized by the advent of massively parallel sequencing, leading to significant advances in genome and transcriptome sequencing of various organisms. Nevertheless, challenges such as accuracy, lack of competitive options and prohibitive costs associated with high throughput parallel short-read sequencing persist. Here, we conduct a comparative analysis between the Element Biosciences AVITI platform and its counterpart the Illumina NextSeq platform, testing DNA and RNA short-read sequencing, as well as synthetic long-read sequencing for single cells and RNA transcripts. Bulk long-read RNA-seq on ERCC spike-in samples by Element Biosciences AVITI platform. Data for all the long reads and data only for full-length transcripts.