Optical maps refine the bread wheat Triticum aestivum cv Chinese Spring genome assembly

This dataset contains a single whole-genome optical map file (.CMAP) for bread wheat (Triticum aestivum) cv Chinese Spring (CS). The methods for constructing this optical map and the properties of this map are described below. The definition of the CMAP format is documented in https://bionanogenomics.com/wp-content/uploads/2017/03/30039-CMAP-File-Format-Specification-Sheet.pdf. 

The extracted ultra-high-molecular weight DNA molecules of CS were labeled with the DLE-1 enzyme (Bionano Genomics, San Diego, CA, USA) and were then stained with the Bionano PrepTM DLS Kit (Bionano Genomics, San Diego, CA, USA). A consensus optical map was de novo assembled with the Assembler tool of the Bionano Solve v3.3 package using significance cutoffs of P < 1 × 10-11 to generate draft consensus contigs, P < 1 × 10-12 for draft consensus contig extension, and P < 1 × 10-16 for final merging of the draft consensus contigs. A recipe of “nonhaplotype”, “noES”, and “noCut” was chosen. The initial ...