Spatial RNA sequencing of bleomycin-treated young and old mice lungs at day 14 and day 28 post injection

We monitored lung fibrosis formation and resolution in young and old mice by generating a series of spatial transcriptomics datasets using the 10x Genomics Visium platform Young (7 weeks) or old (18 months) mice were injected with PBS or bleomycin to induce transient alveolar damages. At the bleomycin-induced fibrotic response peak at day 14 and during the resolution phase at day 28 post injection, their lungs were embedded in OCT and stored at -80°C. OCT blocks were cryosectioned at 10µm thickness on capture areas of Visium 10 Genomics slide.