Single cell RNA-seq data of colonic and small intestinal epithelium from Ecsit fl/fl and Ecsit cKO mice

To investigate the specific role of Ecsit in intestinal homeostasis, we generated Ecsit floxed/Vil1-Cre-ERT2 mouse model, and injected tamoxifen (3mg) intraperitoneally for 5 consecutive days to achieve conditional deletion of Ecsit in the intestinal epithelium at a specific time. These Ecsit fl/fl Vil1-Cre-ERT2 mice (Ecsit cKO) and Control mice (Ecsit fl/fl) mice were euthanized 12 days after the last injection for single cell RNA-seq on small intestinal and colonic epithelium. Overall design: Data of small intestinal epithelium is from two independent experiments, in which experiment, cells were mixed from 3 mice. Data of colonic epithelium is from one experiment in which cells were mixed from 3 mice.