RNA-Seq of Mycobacterium tuberculosis

In this study, deep sequencing analysis of Mycobacterium tuberculosis transcriptomes by RNA-seq allowed the identification of small non-coding RNAs on a genome-wide scale in an increasing number of prokaryotic organisms, and these RNA molecules regulate gene expression in response to changing environments. Here, after generating transcriptomes from exponential phase cultures of Mycobacterium tuberculosis H37Rv and H37Ra, we developed and validated an automated method for genome-wide identification of sRNA candidate-containing regions within RNA-Seq datasets based on the analysis of the characteristics of reads coverage maps to capture the entire cellular transcriptome and subsequent analysis of read coverage map features.