Aspergillus oryzae Raw sequence reads. Aspergillus oryzae

Ergosterol is an unique component in fungal cells, which does not only important for fungi growth and stress responses but also has great economic value. There were little research about ergosterol biosynthesis in Aspergillus oryzae (A. oryzae), a safe filamentous fungus, which has been used for the manufacture of oriental fermented foods. This study tooks advantage of RNA-seq to investage the target genes regulated by ergosterol. The fresh A. oryzae 3.042 spore suspension (about 1×107 spores/ml) was cultured for 2 days on PDA medium covered by cellophane (Solarbio, Beijing, China), and the cellophane with mycelial were transferred into another plate with tebuconazole or terbinafine for 8 hours. Then, the mycelial were collected for RNA-seq. Every treatment and WT were done 2 biological repeats for RNA-seq. And this study identified the differentially expressed genes (DEG) in response to the inhibitors (tebuconazol and terbinafine) treatments.