Wnt target genes up-regulated in Wnt3a-stimulated HCC38 cells and their enrichment in human breast cancer samples.

We assessed the significance of the overlap between the genes that were up-regulated in Wnt3a-stimulated HCC38 (6h, 12h, 24h, and those up-regulated at both 6h and 24h) and those that were strongly expressed in tumors (TNBC, HER2+, LB and LA) (gene nb). We restricted our study to the 11262 genes present on both arrays (HCC38 dataset, tumor dataset). The number of the Wnt target genes that were more strongly expressed is indicated for each tumor comparison. The associated P value is also shown underneath (in Italics). The significance of the overlap between two lists was assessed with the Fisher exact test. As an example, 194 of the 419 Wnt target genes up-regulated in Wnt3a-stimulated HCC38 cells (6h) were more strongly expressed in TNBC than in LA samples (194 of the 3497 genes more strongly expressed genes in TNBC than in LA samples) (P value = 3.5x10-11).Wnt target genes up-regulated in Wnt3a-stimulated HCC38 cells and their enrichment in human breast cancer samples.