Metabolite data demonstrating the response of bay mussels to stormwater, wastewater, and field exposure studies from: Metabolomic signatures of bioenergetic disruption in marine mussels exposed to wastewater, stormwater, and environmental conditions

Nearshore environments are highly impacted by contaminants from
anthropogenic activities, causing long-lasting and far-reaching impacts on
nearshore organism populations. This study relies on metabolomic methods
to understand and quantify the impact of contaminants in stormwater,
wastewater, and field exposure on the reproductive success of native bay
mussels (Mytilus trossulus). Mussels were exposed to known dilutions of
stormwater or wastewater in a laboratory setting, and in a second study,
mussels were deployed to select field sites throughout Puget Sound that
were expected to receive stormwater or wastewater inputs. Hemolymph was
then extracted, and samples were analyzed for metabolite concentrations
using Liquid Chromatography-Mass Spectrometry. Metabolite concentration
data were then analyzed using the MetaboAnalyst platform. Analysis showed
significant disruption to energy pathways in exposed mussels, and a review
of past studies created a strong link between energy metabolite levels and
reproductive success. These results present a strong argument for the use
of energy metabolites as biomarkers of reproductive health in mussels, and
can provide insight into the health of the nearshore ecosystem and its
dependent food webs.