RNA-Seq analysis among dps-disrupted mutants in Deinococcus geothermalis

Transcriptomic analysis was performed in Deinococcus geothermalis wild-type and three mutants including dgeo_0257, dgeo_0281, and double knockout (DK) mutants. dgeo_0281 is a major Dps-coding gene and dgeo_0257 is a novel Dps candidate. Thus, to detecte physiological functions of both Dps-encoded genes, each gene-disrupted mutant and DK mutant was constructed and performed RNA-Seq analysis. This result support better understanding for Dps roles in radiation resistant bacterium through up-regulated and down-regulated genes profile. Overall design: Experimental strains were cultivated to OD600 of 2.0 as an early exponential growth phase in TGY culture medium. The optical density was nomalized to 1.0 value with 0.9% saline and continuously cultivated for 1 hour present or absent of 50 mM H2O2. Then samples were centrifuged and discarded supernatant. The pellets were applied RNA preperation using manufacturing protocols.