Compared the gene expression profiles between the PTL-Her2-CAR-T cells and Her2-CAR-T cells isolated from tumor tissues

To further development of different gene expression between the PTL-Her2-CAR-T cells (knockdown of PD-1, Tim-3, Lag-3 by shRNA-clusters in Her2-CAR-T cells) and Her2-CAR-T cells, we have employed whole genome microarray expression profiling as a discovery platform. SKOV3-bearing NSG mice were injected with various CAR-T cells via tail. At 28 days after CAR-T-cells infusion, mice were euthanized and tumor tissues were resected. The tumor was divided and tumor infiltrating CAR-T cells were separated for RNA-seq.