A BAT-enriched RNA binding protein, Ybx2, regulates cold-induced brown fat activation by stabilizing Pgc1α mRNA

To study the role of Ybx2 in brown fat activity, we performed the RNA-seq data for wildtype and Ybx2 knockout brown fat under room temperature and upon cold exposure as well as for wildtype and Ybx2 knockout brown adipocytes culture from stromal vascular fraction cells. To determine the targets of Ybx2, we performed RNA immunopercipitatoin followed by RNA-seq mRNA profiling of brown fat from wild type and Ybx2 knockout mice, of brown adipocyte culture (day 5) from WT and KO mice, of RNA immunopercipitation samples in brown fat