Specific targeting of plasmids with Argonaute enables genome editing

Prokaryotic Argonaute nuclease induces DNA interference between plasmid and chromosomal DNA to promote genome recombineering Argonaute-associated smDNA from E.coli transformed by plasmids of different constructions was analysed to elucidate the requirements for genomic DNA cleavage by CbAgo during DNA interference and integration of plasmid-encoded sequences into the homologous chromosomal locus Second part of data on DNA interference research. Additional libraries from 2 clones were prepared to show the integration of plasmid-encoded genes into the homologous chromosomal locus promoted by bacterial Argonaut protein