Chemicals targeting Lipid binding domain (LBD) of 38γ

We screened 270,000 compounds from an NCI library for the ability to dock with the non-ATP-binding site of p38γ using virtual screen. The resulting candidate compounds were for cytotoxicity assays to the Hut78 CTCL cell line and identified CSH71 as one of the most promising agents. To show downstream pathway that CSH71-mediated targeting of p38γ, we first isolated RNA and subjected to microarray analysis of treated vs. control cells and observed upregulation of TNF alpha in several top hit after assesments of its downstream pathways.