Data for: Fabrication and characterization of antibacterial coatings using an amphoteric condensed tannin,Tanfloc

This dataset presents comprehensive information on the development and characterization of antibacterial polyelectrolyte multilayer (PEM) coatings incorporating tanfloc, a plant-derived condensed tannin polymer with inherent antimicrobial properties. The dataset encompasses experimental data related to the fabrication of PEMs using tanfloc as either a polyanion or a polycation, expanding the versatility of this amphoteric polymer in PEM coatings. Typically, PEMs are formed by combining a polycation and a polyanion with complementary ionic groups. However, the unique amphoteric nature of tanfloc allows for the creation of PEMs utilizing only one of its functional groups, leaving the other functional group available for imparting antibacterial activity.
The dataset includes details on the assembly of tanfloc-containing PEMs employing various counter-polyelectrolytes, including three poly-anionic glycosaminoglycans with varying charge densities, as well as the polycations N,N,N-trimethyl c..., 1H Nuclear Magnetic Resonance (1H NMR): Tanfloc (TAN) was characterized using 1H NMR spectroscopy, recording spectra on a Bruker Neo US 400 NMR spectrometer operating at 400 MHz.
Dynamic Light Scattering (DLS): Solutions of TAN (tanfloc) with varying pH values (5, 6, 7.4, 8.4, and 9.3) were prepared and subjected to dynamic light scattering (DLS) to determine the hydrodynamic diameter and polydispersity index (PDI). This characterization was performed using a Zetasizer Nano ZS instrument from Malvern.
Fourier-Transform Surface Plasmon Resonance (FT-SPR): The layer-by-layer (LBL) assembly of PEMs on gold-coated glass chips was monitored in real-time using Fourier-Transform Surface Plasmon Resonance (FT-SPR). This involved modifying the gold-coated chips, flowing polycation, polyanion, and rinse solutions, and collecting data using an SPR-100 module coupled to a Nicolet 8700 FT-IR spectrometer.
X-ray Photoelectron Spectroscopy (XPS): Composition analysis of the PEMs was performed using X-..., , # Dataset for Fabrication and Characterization of Antibacterial Coatings using an Amphoteric Condensed Tannin, Tanfloc.

The dataset contains experimental data detailing the development and characterization of antibacterial polyelectrolyte multilayer (PEM) coatings containing tanfloc, a natural condensed tannin polymer, as an amphoteric biomaterial. It includes results from biocompatibility assessments using human adipose-derived stem cells (ADSCs) and antibacterial activity assays against *Pseudomonas aeruginosa* and *Staphylococcus aureus*. Moreover, the dataset provides insights into tanfloc hydrodynamic properties, the real-time assembly of PEMs via Fourier-Transform Surface Plasmon Resonance (FT-SPR), and PEM compositional analysis by X-ray Photoelectron Spectroscopy (XPS). N,N,N-Trimethyl chitosan is also characterized via 1H Nuclear Magnetic Resonance (1H NMR) spectroscopy. Additionally, fluorescence microscopy data quantifies live and dead bacterial adhesion, demonstrating the...