Genotype-by-sequencing in Alpheus snapping shrimp

The formation of the Isthmus of Panama and final closure of the Central American Seaway (CAS) provides an independent calibration point for examining the rate of DNA substitutions. Nuclear sequence data is increasingly being used to complement mitochondrial datasets for phylogenetic and evolutionary investigations; these studies would benefit from information regarding the rate and pattern of DNA substitutions derived from the nuclear genome.To estimate the genome-wide neutral mutation rate, genotype-by-sequencing (GBS) datasets were generated for three transisthmian species pairs in Alpheus snapping shrimp. To our knowledge this is the first use of transisthmian species pairs to calibrate the rate of molecular evolution from GBS data.